Neurotransmitters such as serotonin, dopamine, norepinephrine, and GABA are chemical messengers that regulate mood, sleep, cognition, and pain signaling across the nervous system. Disruptions in their synthesis, release, or reuptake can produce anxiety, persistent low mood, chronic fatigue, insomnia, and impaired concentration. Contributing factors often include prolonged psychological stress, nutritional shortfalls, genetic enzyme variants, and certain prescription medications. Clinical assessment combines structured symptom evaluation with targeted biomarker testing to pinpoint specific neurotransmitter deficits. Management may involve SSRIs, SNRIs, or adjunctive lifestyle measures such as regular aerobic exercise, consistent sleep schedules, and physician-guided nutritional supplementation.